Evaluation of Semen Samples Before and After ‘Swim Up’ Technique with Mitotracker
نویسندگان
چکیده
منابع مشابه
Sperm swim-up: a simple and effective technique of semen processing for intrauterine insemination.
OBJECTIVE To study the impact of sperm swim up technique of seminal processing for the purpose of intrauterine insemination (IUI) in couples presenting with subfertility. METHODS Hundred and twenty-one couples presenting with subfertility underwent 281 cycles of IUI in Combined Military Hospital Kharian, Lahore and PNS SHIFA Karachi from June 2002 to March 2005. Men had prior semen analysis t...
متن کاملSemen preparation by standard swim-up versus swim-up with test yolk buffer incubation in intrauterine insemination: a randomized study.
In order to compare the standard swim-up semen preparation with and without test yolk buffer (TYB) incubation in intrauterine insemination (IUI), we conducted a prospective multicentre randomized trial. A total of 121 infertile couples with male factor (n = 52) or unexplained infertility (n = 69) was randomly assigned to two groups following ovulation induction. Semen was prepared by standard s...
متن کاملEvaluation of the semen swim-up method for bovine sperm RNA extraction.
Isolation of high-quality RNA is important for assessing sperm gene expression, and semen purification methods may affect the integrity of the isolated RNA. This study evaluated the effectiveness of the sperm swim-up method for seminal RNA isolation. Frozen semen samples in straws from three bulls of proven fertility were purified by the swim-up method. RNA extraction was carried out using the ...
متن کاملProfound Transcriptomic Differences Found between Sperm Samples from Sperm Donors vs. Patients Undergoing Assisted Reproduction Techniques Tends to Disappear after Swim-up Sperm Preparation Technique
Background Although spermatozoa delivers its RNA to oocytes at fertilization, its biological role is not well characterized. Our purpose was to identify the genes differentially and exclusively expressed in sperm samples both before and after the swim-up process in control donors and infertile males with the purpose to identify their functional significance in male fertility. MaterialsAndMethod...
متن کاملSemen quality before and after gonadotoxic treatment.
BACKGROUND The aim of this study was to analyse the semen quality of patients before and after gonadotoxic therapy. PATIENTS AND METHODS We evaluated semen quality in 314 patients over a 26 year period. The diagnostic categories were leukaemia (n = 13); lymphoma (n = 128); testicular cancer (n = 102); benign conditions (n = 13); and other malignant neoplasms (n = 58). The degree of azoospermi...
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ژورنال
عنوان ژورنال: Bangladesh Journal of Medical Science
سال: 2019
ISSN: 2076-0299,2223-4721
DOI: 10.3329/bjms.v18i3.41614